A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians.

Dev Cell
Authors
Keywords
Abstract

Regeneration starts with injury. Yet how injuries affect gene expression in different cell types and how distinct injuries differ in gene expression remain unclear. We defined the transcriptomes of major cell types of planarians--flatworms that regenerate from nearly any injury--and identified 1,214 tissue-specific markers across 13 cell types. RNA sequencing on 619 single cells revealed that wound-induced genes were expressed either in nearly all cell types or specifically in one of three cell types (stem cells, muscle, or epidermis). Time course experiments following different injuries indicated that a generic wound response is activated with any injury regardless of the regenerative outcome. Only one gene, notum, was differentially expressed early between anterior- and posterior-facing wounds. Injury-specific transcriptional responses emerged 30 hr after injury, involving context-dependent patterning and stem-cell-specialization genes. The regenerative requirement of every injury is different; however, our work demonstrates that all injuries start with a common transcriptional response.

Year of Publication
2015
Journal
Dev Cell
Volume
35
Issue
5
Pages
632-45
Date Published
2015 Dec 07
ISSN
1878-1551
URL
DOI
10.1016/j.devcel.2015.11.004
PubMed ID
26651295
PubMed Central ID
PMC4817857
Links
Grant list
Howard Hughes Medical Institute / United States
F32 HD075541 / HD / NICHD NIH HHS / United States
R01 GM080639 / GM / NIGMS NIH HHS / United States
R01GM080639 / GM / NIGMS NIH HHS / United States