History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains.

Virology
Authors
Keywords
Abstract

A collection of genomic DNA sequences of herpes simplex virus (HSV) strains has been defined and analyzed, and some information is available about genomic stability upon limited passage of viruses in culture. The nature of genomic change upon extensive laboratory passage remains to be determined. In this report we review the history of the HSV-1 KOS laboratory strain and the related KOS1.1 laboratory sub-strain, also called KOS (M), and determine the complete genomic sequence of an early passage stock of the KOS laboratory sub-strain and a laboratory stock of the KOS1.1 sub-strain. The genomes of the two sub-strains are highly similar with only five coding changes, 20 non-coding changes, and about twenty non-ORF sequence changes. The coding changes could potentially explain the KOS1.1 phenotypic properties of increased replication at high temperature and reduced neuroinvasiveness. The study also provides sequence markers to define the provenance of specific laboratory KOS virus stocks.

Year of Publication
2016
Journal
Virology
Volume
487
Pages
215-21
Date Published
2016 Jan
ISSN
1096-0341
URL
DOI
10.1016/j.virol.2015.09.026
PubMed ID
26547038
PubMed Central ID
PMC4679709
Links
Grant list
R56 AI057552 / AI / NIAID NIH HHS / United States
HHSN272200900018C / AI / NIAID NIH HHS / United States
AI057552 / AI / NIAID NIH HHS / United States
P01 AI098681 / AI / NIAID NIH HHS / United States
R01 AI057552 / AI / NIAID NIH HHS / United States
AI098681 / AI / NIAID NIH HHS / United States
R01 AI030612 / AI / NIAID NIH HHS / United States
U19 AI110818 / AI / NIAID NIH HHS / United States