Version 4.0, 21 August 2007

Nature biotechnology
Authors
Abstract

We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.

Year of Publication
2015
Journal
Nature biotechnology
Date Published
2015/10/05
ISSN
1087-0156
URL
DOI
10.1038/nbt.3390
Links