Version 4.0, 21 August 2007
Nature biotechnology
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Abstract | We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells. |
Year of Publication | 2015
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Journal | Nature biotechnology
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Date Published | 2015/10/05
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ISSN | 1087-0156
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URL | |
DOI | 10.1038/nbt.3390
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