High-throughput identification of phage-derived imaging agents.

Molecular imaging
Authors
Abstract

The use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of "hits" obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis method to identify and display hits from phage-display experiments and high-throughput enzyme-linked immunosorbent assay screens. We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries.

Year of Publication
2006
Journal
Molecular imaging
Volume
5
Issue
1
Pages
24-30
Date Published
2006/01/01
ISSN
1535-3508
DOI
10.2310/7290.2006.00003
PubMed ID
16779967
Links