Spatiotemporal expression and transcriptional perturbations by long noncoding RNAs in the mouse brain.

Proc Natl Acad Sci U S A
Authors
Keywords
Abstract

Long noncoding RNAs (lncRNAs) have been implicated in numerous cellular processes including brain development. However, the in vivo expression dynamics and molecular pathways regulated by these loci are not well understood. Here, we leveraged a cohort of 13 lncRNAnull mutant mouse models to investigate the spatiotemporal expression of lncRNAs in the developing and adult brain and the transcriptome alterations resulting from the loss of these lncRNA loci. We show that several lncRNAs are differentially expressed both in time and space, with some presenting highly restricted expression in only selected brain regions. We further demonstrate altered regulation of genes for a large variety of cellular pathways and processes upon deletion of the lncRNA loci. Finally, we found that 4 of the 13 lncRNAs significantly affect the expression of several neighboring proteincoding genes in a cis-like manner. By providing insight into the endogenous expression patterns and the transcriptional perturbations caused by deletion of the lncRNA locus in the developing and postnatal mammalian brain, these data provide a resource to facilitate future examination of the specific functional relevance of these genes in neural development, brain function, and disease.

Year of Publication
2015
Journal
Proc Natl Acad Sci U S A
Volume
112
Issue
22
Pages
6855-62
Date Published
2015 Jun 02
ISSN
1091-6490
URL
DOI
10.1073/pnas.1411263112
PubMed ID
26034286
PubMed Central ID
PMC4460505
Links
Grant list
R01MH102416-0 / MH / NIMH NIH HHS / United States
R01 NS062849 / NS / NINDS NIH HHS / United States
R01 MH101268 / MH / NIMH NIH HHS / United States
R01 MH102416 / MH / NIMH NIH HHS / United States
R01MH101268 / MH / NIMH NIH HHS / United States
R01NS062849 / NS / NINDS NIH HHS / United States