DNA copy number analysis of metastatic urothelial carcinoma with comparison to primary tumors.
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Abstract | BACKGROUND: To date, there have been no reports characterizing the genome-wide somatic DNA chromosomal copy-number alteration landscape in metastatic urothelial carcinoma. We sought to characterize the DNA copy-number profile in a cohort of metastatic samples and compare them to a cohort of primary urothelial carcinoma samples in order to identify changes that are associated with progression from primary to metastatic disease. METHODS: Using molecular inversion probe array analysis we compared genome-wide chromosomal copy-number alterations between 30 metastatic and 29 primary UC samples. Whole transcriptome RNA-Seq analysis was also performed in primary and matched metastatic samples which was available for 9 patients. RESULTS: Based on a focused analysis of 32 genes in which alterations may be clinically actionable, there were significantly more amplifications/deletions in metastases (8.6% vs 4.5%, p CONCLUSIONS: The discordance in alterations between primary and metastatic tissue may be of clinical relevance in the era of genomically directed precision cancer medicine. |
Year of Publication | 2015
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Journal | BMC Cancer
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Volume | 15
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Pages | 242
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Date Published | 2015 Apr 09
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ISSN | 1471-2407
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DOI | 10.1186/s12885-015-1192-2
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PubMed ID | 25886454
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PubMed Central ID | PMC4392457
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