High-throughput functional genomics using CRISPR-Cas9.
Nat Rev Genet
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Abstract | Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges. |
Year of Publication | 2015
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Journal | Nat Rev Genet
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Volume | 16
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Issue | 5
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Pages | 299-311
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Date Published | 2015 May
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ISSN | 1471-0064
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URL | |
DOI | 10.1038/nrg3899
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PubMed ID | 25854182
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PubMed Central ID | PMC4503232
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Grant list | DP1‑MH100706 / DP / NCCDPHP CDC HHS / United States
K99 HG008171 / HG / NHGRI NIH HHS / United States
K99‑HG008171 / HG / NHGRI NIH HHS / United States
DP1 MH100706 / MH / NIMH NIH HHS / United States
R01‑NS07312401 / NS / NINDS NIH HHS / United States
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