Immunogenetics. Dynamic profiling of the protein life cycle in response to pathogens.

Science
Authors
Keywords
Abstract

Protein expression is regulated by the production and degradation of messenger RNAs (mRNAs) and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics so as to build a quantitative genomic model of the differential regulation of gene expression in lipopolysaccharide-stimulated mouse dendritic cells. Changes in mRNA abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for more than half of the absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction for newly activated cellular functions and by protein life-cycle changes for remodeling of preexisting functions.

Year of Publication
2015
Journal
Science
Volume
347
Issue
6226
Pages
1259038
Date Published
2015 Mar 06
ISSN
1095-9203
URL
DOI
10.1126/science.1259038
PubMed ID
25745177
PubMed Central ID
PMC4506746
Links
Grant list
F32 HD075541 / HD / NICHD NIH HHS / United States
T32 GM008284 / GM / NIGMS NIH HHS / United States
T32 HG002295 / HG / NHGRI NIH HHS / United States
Howard Hughes Medical Institute / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States