Scientific Publications

Allele-specific methylation occurs at genetic variants associated with complex disease.

Publication TypeJournal Article
AuthorsHutchinson, JN, Raj T., Fagerness J., Stahl E., Viloria FT, Gimelbrant A., Seddon J., Daly M., Chess A., and Plenge R.
AbstractWe hypothesize that the phenomenon of allele-specific methylation (ASM) may underlie the phenotypic effects of multiple variants identified by Genome-Wide Association studies (GWAS). We evaluate ASM in a human population and document its genome-wide patterns in an initial screen at up to 380,678 sites within the genome, or up to 5% of the total genomic CpGs. We show that while substantial inter-individual variation exists, 5% of assessed sites show evidence of ASM in at least six samples; the majority of these events (81%) are under genetic influence. Many of these cis-regulated ASM variants are also eQTLs in peripheral blood mononuclear cells and monocytes and/or in high linkage-disequilibrium with variants linked to complex disease. Finally, focusing on autoimmune phenotypes, we extend this initial screen to confirm the association of cis-regulated ASM with multiple complex disease-associated variants in an independent population using next-generation bisulfite sequencing. These four variants are implicated in complex phenotypes such as ulcerative colitis and AIDS progression disease (rs10491434), Celiac disease (rs2762051), Crohn's disease, IgA nephropathy and early-onset inflammatory bowel disease (rs713875) and height (rs6569648). Our results suggest cis-regulated ASM may provide a mechanistic link between the non-coding genetic changes and phenotypic variation observed in these diseases and further suggests a route to integrating DNA methylation status with GWAS results.
Year of Publication2014
JournalPloS one
Volume9
Issue6
Pagese98464
Date Published (YYYY/MM/DD)2014/01/01
DOI10.1371/journal.pone.0098464
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/24911414?dopt=Abstract