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Targeted genomic rearrangements using CRISPR/Cas technology.
|Publication Type||Journal Article|
|Authors||Choi, PS, and Meyerson M.|
|Abstract||Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.|
|Year of Publication||2014|
|Date Published (YYYY/MM/DD)||2014/04/24|