Targeted genomic rearrangements using CRISPR/Cas technology.
Nat Commun
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Abstract | Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements. |
Year of Publication | 2014
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Journal | Nat Commun
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Volume | 5
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Pages | 3728
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Date Published | 2014 Apr 24
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ISSN | 2041-1723
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URL | |
DOI | 10.1038/ncomms4728
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PubMed ID | 24759083
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PubMed Central ID | PMC4170920
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Grant list | R01 CA109038 / CA / NCI NIH HHS / United States
NCI R01CA109038 / CA / NCI NIH HHS / United States
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