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ZBED6 Modulates the Transcription of Myogenic Genes in Mouse Myoblast Cells.
|Publication Type||Journal Article|
|Authors||Jiang, L., Wallerman O., Younis S., Rubin CJ, Gilbert ER, Sundström E., Ghazal A., Zhang X., Wang L., Mikkelsen TS, Andersson G., and Andersson L.|
|Abstract||ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differentially expressed after Zbed6-silencing. The most significantly enriched GO term was muscle protein and contractile fiber, which was consistent with increased myotube formation. Twenty small nucleolar RNAs all showed increased expression after Zbed6-silencing. The co-localization of histone marks and ZBED6 binding sites and the effect of Zbed6-silencing on distribution of histone marks was evaluated by ChIP-seq analysis. There was a strong association between ZBED6 binding sites and the H3K4me3, H3K4me2 and H3K27ac modifications, which are usually found at active promoters, but no association with the repressive mark H3K27me3. Zbed6-silencing led to increased enrichment of active marks at myogenic genes, in agreement with the RNA-seq findings. We propose that ZBED6 preferentially binds to active promoters and modulates transcriptional activity without recruiting repressive histone modifications.|
|Year of Publication||2014|
|Date Published (YYYY/MM/DD)||2014/01/01|