Scientific Publications

RNA-Guided Genome Editing of Mammalian Cells.

Publication TypeJournal Article
AuthorsPyzocha, NK, Ran FA, Hsu PD, and Zhang F.
AbstractThe microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.
Year of Publication2014
JournalMethods in molecular biology (Clifton, N.J.)
Volume1114
Pages269-77
Date Published (YYYY/MM/DD)2014/01/01
ISSN Number1064-3745
DOI10.1007/978-1-62703-761-7_17
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/24557909?dopt=Abstract