Scientific Publications

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins.

Publication TypeJournal Article
AuthorsKennedy, JJ, Abbatiello SE, Kim K., Yan P., Whiteaker JR, Lin C., Kim JS, Zhang Y., Wang X., Ivey RG, Zhao L., Min H., Lee Y., Yu MH, Yang EG, Lee C., Wang P., Rodriguez H., Kim Y., Carr SA, and Paulovich AG
AbstractMultiple reaction monitoring (MRM) mass spectrometry has been successfully applied to monitor targeted proteins in biological specimens, raising the possibility that assays could be configured to measure all human proteins. We report the results of a pilot study designed to test the feasibility of a large-scale, international effort for MRM assay generation. We have configured, validated across three laboratories and made publicly available as a resource to the community 645 novel MRM assays representing 319 proteins expressed in human breast cancer. Assays were multiplexed in groups of >150 peptides and deployed to quantify endogenous analytes in a panel of breast cancer-related cell lines. The median assay precision was 5.4%, with high interlaboratory correlation (R(2) > 0.96). Peptide measurements in breast cancer cell lines were able to discriminate among molecular subtypes and identify genome-driven changes in the cancer proteome. These results establish the feasibility of a large-scale effort to develop an MRM assay resource.
Year of Publication2014
JournalNature methods
Volume11
Issue2
Pages149-55
Date Published (YYYY/MM/DD)2014/02/01
ISSN Number1548-7091
DOI10.1038/nmeth.2763
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/24317253?dopt=Abstract