Multiplex cytological profiling assay to measure diverse cellular states.

PLoS One
Authors
Keywords
Abstract

Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

Year of Publication
2013
Journal
PLoS One
Volume
8
Issue
12
Pages
e80999
Date Published
2013
ISSN
1932-6203
URL
DOI
10.1371/journal.pone.0080999
PubMed ID
24312513
PubMed Central ID
PMC3847047
Links
Grant list
RL1HG004671 / HG / NHGRI NIH HHS / United States
U54 HG005032 / HG / NHGRI NIH HHS / United States