Scientific Publications

MetaRef: a pan-genomic database for comparative and community microbial genomics.

Publication TypeJournal Article
AuthorsHuang, K., Brady A., Mahurkar A., White O., Gevers D., Huttenhower C., and Segata N.
AbstractMicrobial genome sequencing is one of the longest-standing areas of biological database development, but high-throughput, low-cost technologies have increased its throughput to an unprecedented number of new genomes per year. Several thousand microbial genomes are now available, necessitating new approaches to organizing information on gene function, phylogeny and microbial taxonomy to facilitate downstream biological interpretation. MetaRef, available at http://metaref.org, is a novel online resource systematically cataloguing a comprehensive pan-genome of all microbial clades with sequenced isolates. It organizes currently available draft and finished bacterial and archaeal genomes into quality-controlled clades, reports all core and pan gene families at multiple levels in the resulting taxonomy, and it annotates families' conservation, phylogeny and consensus functional information. MetaRef also provides a comprehensive non-redundant reference gene catalogue for metagenomic studies, including the abundance and prevalence of all gene families in the >700 shotgun metagenomic samples of the Human Microbiome Project. This constitutes a systematic mapping of clade-specific microbial functions within the healthy human microbiome across multiple body sites and can be used as reference for identifying potential functional biomarkers in disease-associate microbiomes. MetaRef provides all information both as an online browsable resource and as downloadable sequences and tabular data files that can be used for subsequent offline studies.
Year of Publication2013
JournalNucleic acids research
Date Published (YYYY/MM/DD)2013/11/06
ISSN Number0305-1048
DOI10.1093/nar/gkt1078
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/24203705?dopt=Abstract