Semiconductor-based DNA sequencing of histone modification states.

Nat Commun
Authors
Keywords
Abstract

The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.

Year of Publication
2013
Journal
Nat Commun
Volume
4
Pages
2672
Date Published
2013
ISSN
2041-1723
URL
DOI
10.1038/ncomms3672
PubMed ID
24157732
PubMed Central ID
PMC3917140
Links
Grant list
U54 HG003067 / HG / NHGRI NIH HHS / United States
P30 DK043351 / DK / NIDDK NIH HHS / United States
U01 CA1411508 / CA / NCI NIH HHS / United States
F32 CA168253 / CA / NCI NIH HHS / United States
U54HG003067 / HG / NHGRI NIH HHS / United States
DP1OD003958-01 / OD / NIH HHS / United States
R01 CA093947 / CA / NCI NIH HHS / United States
Howard Hughes Medical Institute / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
1P50HG006193-01 / HG / NHGRI NIH HHS / United States
P50 CA093459 / CA / NCI NIH HHS / United States
U01 CA141508 / CA / NCI NIH HHS / United States
R01 CA93947 / CA / NCI NIH HHS / United States
DP1 OD003958 / OD / NIH HHS / United States