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Magnetic barcode assay for genetic detection of pathogens.
|Publication Type||Journal Article|
|Authors||Liong, M., Hoang AN, Chung J., Gural N., Ford CB, Min C., Shah RR, Ahmad R., Fernandez-Suarez M., Fortune SM, Toner M., Lee H., and Weissleder R.|
|Abstract||The task of rapidly identifying patients infected with Mycobacterium tuberculosis in resource-constrained environments remains a challenge. A sensitive and robust platform that does not require bacterial isolation or culture is critical in making informed diagnostic and therapeutic decisions. Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labelled by magnetic nanoprobes and detected by nuclear magnetic resonance. All components are integrated into a single, small fluidic cartridge for streamlined on-chip operation. We use this platform to detect M. tuberculosis and identify drug-resistance strains from mechanically processed sputum samples within 2.5 h. The specificity of the assay is confirmed by detecting a panel of clinically relevant non-M. tuberculosis bacteria, and the clinical utility is demonstrated by the measurements in M. tuberculosis-positive patient specimens. Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput and low-cost platform for point-of-care diagnostics.|
|Year of Publication||2013|
|Date Published (YYYY/MM/DD)||2013/01/01|