Scientific Publications

Integrated proteomic analysis of post-translational modifications by serial enrichment.

Publication TypeJournal Article
AuthorsMertins, P., Qiao JW, Patel J., Udeshi ND, Clauser KR, Mani D. R., Burgess MW, Gillette MA, Jaffe JD, and Carr SA
AbstractWe report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.
Year of Publication2013
JournalNature methods
Volume10
Issue7
Pages634-7
Date Published (YYYY/MM/DD)2013/07/01
ISSN Number1548-7091
DOI10.1038/nmeth.2518
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/23749302?dopt=Abstract