Scientific Publications

Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: IV. viral proteinase slides along DNA to locate and process its substrates.

Publication TypeJournal Article
AuthorsBlainey, PC, Graziano V., Pérez-Berná AJ, McGrath WJ, Flint SJ, San Martín C., Xie XS, and Mangel WF
AbstractPrecursor proteins used in the assembly of adenovirus virions must be processed by the virally encoded adenovirus proteinase (AVP) before the virus particle becomes infectious. An activated adenovirus proteinase, the AVP-pVIc complex, was shown to slide along viral DNA with an extremely fast one-dimensional diffusion constant, 21.0 ± 1.9 × 10(6) bp(2)/s. In principle, one-dimensional diffusion can provide a means for DNA-bound proteinases to locate and process DNA-bound substrates. Here, we show that this is correct. In vitro, AVP-pVIc complexes processed a purified virion precursor protein in a DNA-dependent reaction; in a quasi in vivo environment, heat-disrupted ts-1 virions, AVP-pVIc complexes processed five different precursor proteins in DNA-dependent reactions. Sliding of AVP-pVIc complexes along DNA illustrates a new biochemical mechanism by which a proteinase can locate its substrates, represents a new paradigm for virion maturation, and reveals a new way of exploiting the surface of DNA.
Year of Publication2013
JournalThe Journal of biological chemistry
Volume288
Issue3
Pages2092-102
Date Published (YYYY/MM/DD)2013/01/18
ISSN Number0021-9258
DOI10.1074/jbc.M112.407460
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/23043138?dopt=Abstract