Scientific Publications

Genomics of Loa loa, a Wolbachia-free filarial parasite of humans.

Publication TypeJournal Article
AuthorsDesjardins, CA, Cerqueira GC, Goldberg JM, Dunning Hotopp JC, Haas BJ, Zucker J., Ribeiro JM, Saif S., Levin JZ, Fan L., Zeng Q., Russ C., Wortman JR, Fink DL, Birren BW, and Nutman TB
AbstractLoa loa, the African eyeworm, is a major filarial pathogen of humans. Unlike most filariae, L. loa does not contain the obligate intracellular Wolbachia endosymbiont. We describe the 91.4-Mb genome of L. loa and that of the related filarial parasite Wuchereria bancrofti and predict 14,907 L. loa genes on the basis of microfilarial RNA sequencing. By comparing these genomes to that of another filarial parasite, Brugia malayi, and to those of several other nematodes, we demonstrate synteny among filariae but not with nonparasitic nematodes. The L. loa genome encodes many immunologically relevant genes, as well as protein kinases targeted by drugs currently approved for use in humans. Despite lacking Wolbachia, L. loa shows no new metabolic synthesis or transport capabilities compared to other filariae. These results suggest that the role of Wolbachia in filarial biology is more subtle than previously thought and reveal marked differences between parasitic and nonparasitic nematodes.
Year of Publication2013
JournalNature genetics
Volume45
Issue5
Pages495-500
Date Published (YYYY/MM/DD)2013/04/26
ISSN Number1061-4036
DOI10.1038/ng.2585
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/23525074?dopt=Abstract