The future is now: single-cell genomics of bacteria and archaea.
Authors | |
Keywords | |
Abstract | Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a road map for future studies in single-cell genomics of environmental microorganisms. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions, and fundamental aspects of microbial population biology. |
Year of Publication | 2013
|
Journal | FEMS Microbiol Rev
|
Volume | 37
|
Issue | 3
|
Pages | 407-27
|
Date Published | 2013 May
|
ISSN | 1574-6976
|
URL | |
DOI | 10.1111/1574-6976.12015
|
PubMed ID | 23298390
|
PubMed Central ID | PMC3878092
|
Links | |
Grant list | R01 HG004863 / HG / NHGRI NIH HHS / United States
|