Mouse ooplasm confers context-specific reprogramming capacity.

Nat Genet
Authors
Keywords
Abstract

Enucleated oocytes have the distinctive ability to reprogram somatic nuclei back to totipotency. Here, we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer, such as germline-associated promoters, as well as unique limitations that include certain repetitive element classes.

Year of Publication
2012
Journal
Nat Genet
Volume
44
Issue
9
Pages
978-80
Date Published
2012 Sep
ISSN
1546-1718
URL
DOI
10.1038/ng.2382
PubMed ID
22902786
PubMed Central ID
PMC3432711
Links
Grant list
P01 GM099117 / GM / NIGMS NIH HHS / United States
U01ES017155 / ES / NIEHS NIH HHS / United States
5DP1OD003958 / OD / NIH HHS / United States
DP1 CA174427 / CA / NCI NIH HHS / United States
P01GM099117 / GM / NIGMS NIH HHS / United States
Howard Hughes Medical Institute / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
U01 ES017155 / ES / NIEHS NIH HHS / United States
DP1 OD003958 / OD / NIH HHS / United States
1P50HG006193 / HG / NHGRI NIH HHS / United States