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Activity-dependent A-to-I RNA Editing in Rat Cortical Neurons.

Publication TypeJournal Article
AuthorsSanjana, NE, Levanon EY, Hueske EA, Ambrose JM, and Li JB
AbstractChanges in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hours of high potassium depolarization but not after 1 hour and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.
Year of Publication2012
JournalGenetics
Date Published (YYYY/MM/DD)2012/06/19
ISSN Number0016-6731
DOI10.1534/genetics.112.141200
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/22714409?dopt=Abstract