An image analysis toolbox for high-throughput C. elegans assays.

Nat Methods
Authors
Keywords
Abstract

We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available through the open-source CellProfiler project and enables objective scoring of whole-worm high-throughput image-based assays of C. elegans for the study of diverse biological pathways that are relevant to human disease.

Year of Publication
2012
Journal
Nat Methods
Volume
9
Issue
7
Pages
714-6
Date Published
2012 Apr 22
ISSN
1548-7105
URL
DOI
10.1038/nmeth.1984
PubMed ID
22522656
PubMed Central ID
PMC3433711
Links
Grant list
P30 DK043351 / DK / NIDDK NIH HHS / United States
U54 EB005149 / EB / NIBIB NIH HHS / United States
K99 DK087928 / DK / NIDDK NIH HHS / United States
R01 DK070147 / DK / NIDDK NIH HHS / United States
R01 AI085581 / AI / NIAID NIH HHS / United States
R01 GM089652 / GM / NIGMS NIH HHS / United States
R01 GM095672 / GM / NIGMS NIH HHS / United States
P01 AI083214 / AI / NIAID NIH HHS / United States
R01 AI072508 / AI / NIAID NIH HHS / United States
K99DK087928 / DK / NIDDK NIH HHS / United States
R01 GM101056 / GM / NIGMS NIH HHS / United States