RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities.
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Abstract | With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to diagnosing the presence and drug-susceptibility of infectious diseases based on direct detection of RNA from clinical samples. We demonstrate that species-specific RNA signatures can be used to identify a broad spectrum of infectious agents, including bacteria, viruses, yeast, and parasites. Moreover, we show that the behavior of a small set of bacterial transcripts after a brief antibiotic pulse can rapidly differentiate drug-susceptible and -resistant organisms and that these measurements can be made directly from clinical materials. Thus, transcriptional signatures could form the basis of a uniform diagnostic platform applicable across a broad range of infectious agents. |
Year of Publication | 2012
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Journal | Proc Natl Acad Sci U S A
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Volume | 109
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Issue | 16
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Pages | 6217-22
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Date Published | 2012 Apr 17
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ISSN | 1091-6490
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URL | |
DOI | 10.1073/pnas.1119540109
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PubMed ID | 22474362
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PubMed Central ID | PMC3341018
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Grant list | T32 AI007061 / AI / NIAID NIH HHS / United States
K08 AI080944 / AI / NIAID NIH HHS / United States
F32 AI081477 / AI / NIAID NIH HHS / United States
R01 AI063106 / AI / NIAID NIH HHS / United States
U54 AI057159 / AI / NIAID NIH HHS / United States
AI 063106 / AI / NIAID NIH HHS / United States
AI 081477 / AI / NIAID NIH HHS / United States
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