Scientific Publications

A human proteome detection and quantitation project.

Publication TypeJournal Article
AuthorsAnderson, NL, Anderson NG, Pearson TW, Borchers CH, Paulovich AG, Patterson SD, Gillette M., Aebersold R., and Carr SA
AbstractThe lack of sensitive, specific, multiplexable assays for most human proteins is the major technical barrier impeding development of candidate biomarkers into clinically useful tests. Recent progress in mass spectrometry-based assays for proteotypic peptides, particularly those with specific affinity peptide enrichment, offers a systematic and economical path to comprehensive quantitative coverage of the human proteome. A complete suite of assays, e.g. two peptides from the protein product of each of the approximately 20,500 human genes (here termed the human Proteome Detection and Quantitation project), would enable rapid and systematic verification of candidate biomarkers and lay a quantitative foundation for subsequent efforts to define the larger universe of splice variants, post-translational modifications, protein-protein interactions, and tissue localization.
Year of Publication2009
JournalMolecular & cellular proteomics : MCP
Volume8
Issue5
Pages883-6
Date Published (YYYY/MM/DD)2009/05/01
ISSN Number1535-9476
DOI10.1074/mcp.R800015-MCP200
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/19131327?dopt=Abstract