Scientific Publications

Detecting protein-small molecule interactions using fluorous small-molecule microarrays.

Publication TypeJournal Article
AuthorsVegas, AJ, and Koehler AN
AbstractGeneral binding assays involving microarrays of small molecules can be used to identify small molecule ligands for nearly any protein, even in the absence of knowledge about protein structure or function. Several suitable methods for manufacturing small molecule microarrays (SMMs) exist and different immobilization methods may be more or less preferable for any given application. Here, we describe a protocol for noncovalent and homogenous capture of small molecules using fluorous interactions between small molecules containing fluorocarbon tags and fluorocarbon-coated glass surfaces. These arrays are especially useful for applications that require display of compounds in a specific orientation such as screening biased libraries.
Year of Publication2010
JournalMethods in molecular biology (Clifton, N.J.)
Volume669
Pages43-55
Date Published (YYYY/MM/DD)2010/01/01
ISSN Number1064-3745
DOI10.1007/978-1-60761-845-4_4
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/20857356?dopt=Abstract