Genetic dissection of complex traits with chromosome substitution strains of mice.

Science
Authors
Keywords
Abstract

Chromosome substitution strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, and behavioral processes. Here, we report the construction of a complete CSS panel for a vertebrate species. The CSS panel consists of 22 mouse strains, each of which carries a single chromosome substituted from a donor strain (A/J) onto a common host background (C57BL/6J). A survey of 53 traits revealed evidence for 150 QTLs affecting serum levels of sterols and amino acids, diet-induced obesity, and anxiety. These results demonstrate that CSSs greatly facilitate the detection and identification of genes that control the wide diversity of naturally occurring phenotypic variation in the A/J and C57BL/6J inbred strains.

Year of Publication
2004
Journal
Science
Volume
304
Issue
5669
Pages
445-8
Date Published
2004 Apr 16
ISSN
1095-9203
DOI
10.1126/science.1093139
PubMed ID
15031436
Links
Grant list
GM07250 / GM / NIGMS NIH HHS / United States
HD07518 / HD / NICHD NIH HHS / United States
RR12305 / RR / NCRR NIH HHS / United States