Scientific Publications

Rpd3p relocation mediates a transcriptional response to rapamycin in yeast.

Publication TypeJournal Article
AuthorsHumphrey, EL, Shamji AF, Bernstein BE, and Schreiber SL
AbstractTreating yeast cells with rapamycin, a small molecule that inhibits the TOR proteins, leads to the repression of many genes. Consistent with prior studies, we find that RPD3, which encodes a histone deacetylase (HDAC), is required for repression upon rapamycin treatment. To elucidate the mechanism underlying RPD3-mediated repression, we screened all promoters in yeast for occupancy by Rpd3p before and after treatment with rapamycin. We find that Rpd3p binds to the promoters of rapamycin-repressible genes only following treatment. These data conflict with a previously proposed model suggesting that Rpd3p is constitutively bound to rapamycin-repressible genes and becomes active only after a stimulus such as treatment with rapamycin. Rather, the comprehensive analysis presented here strongly supports a model in which recruitment of Rpd3p to gene promoters is a regulated step in the control of gene repression.
Year of Publication2004
JournalChemistry & biology
Volume11
Issue3
Pages295-9
Date Published (YYYY/MM/DD)2004/03/01
ISSN Number1074-5521
DOI10.1016/j.chembiol.2004.03.001
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/15123258?dopt=Abstract