Recent Broad Publications
- Small-Molecule Inhibitors of Cytokine-Mediated STAT1 Signal Transduction in β-Cells with Improved Aqueous Solubility.
Read More / View Supplemental Materials - Transcriptional and Epigenetic Dynamics during Specification of Human Embryonic Stem Cells.
Read More / View Supplemental Materials - Entering the age of whole-exome sequencing in rheumatic diseases: Novel insights into disease pathogenicity.
Read More / View Supplemental Materials - Young and old genetically heterogeneous HET3 mice on a rapamycin diet are glucose intolerant but insulin sensitive.
Read More / View Supplemental Materials - miR-155 in Acute Myeloid Leukemia: Not Merely a Prognostic Marker?
Read More / View Supplemental Materials
Scientific Publications
< Back to Publications
Complex phenotypic assays in high-throughput screening.
| Publication Type | Journal Article |
| Authors | Clemons, PA |
| Abstract | High-throughput screening (HTS), systematically testing thousands of small molecules to find candidates for lead optimization, primarily involves exposure of purified proteins to arrayed collections of small molecules. More complex phenotypic assays, such as cell-based or whole-organism assays, traditionally have flanked HTS, preceding it to validate new therapeutic targets, and following it to characterize new lead compounds in cellular contexts. Recently, however, cell- and organism-based phenotypic assays have increasingly been adopted as a primary screening platform for annotating small molecules. |
| Year of Publication | 2004 |
| Journal | Current opinion in chemical biology |
| Volume | 8 |
| Issue | 3 |
| Pages | 334-8 |
| Date Published (YYYY/MM/DD) | 2004/06/01 |
| ISSN Number | 1367-5931 |
| DOI | 10.1016/j.cbpa.2004.04.002 |
| PubMed | http://www.ncbi.nlm.nih.gov/pubmed/15183333?dopt=Abstract |
Share This
- Tweet
