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Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.
|Publication Type||Journal Article|
|Authors||Michalkiewicz, M., Michalkiewicz T., Ettinger RA, Rutledge EA, Fuller JM, Moralejo DH, Van Yserloo B., MacMurray AJ, Kwitek AE, Jacob HJ, Lander E. S., and Lernmark A.|
|Abstract||A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.|
|Year of Publication||2004|
|Date Published (YYYY/MM/DD)||2004/10/04|