Scientific Publications

Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae.

Publication TypeJournal Article
AuthorsGalagan, JE, Calvo SE, Cuomo C., Ma LJ, Wortman JR, Batzoglou S., Lee SI, Baştürkmen M., Spevak CC, Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M., Butler J., Purcell S., Harris S., Braus GH, Draht O., Busch S., D'Enfert C., Bouchier C., Goldman GH, Bell-Pedersen D., Griffiths-Jones S., Doonan JH, Yu J., Vienken K., Pain A., Freitag M., Selker EU, Archer DB, Peñalva MA, Oakley BR, Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman WC, Denning DW, Caddick M., Hynes M., Paoletti M., Fischer R., Miller B., Dyer P., Sachs MS, Osmani SA, and Birren BW
AbstractThe aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence conservation revealed over 5,000 non-coding regions actively conserved across all three species. Within these regions, we identified potential functional elements including a previously uncharacterized TPP riboswitch and motifs suggesting regulation in filamentous fungi by Puf family genes. We further obtained comparative and experimental evidence indicating widespread translational regulation by upstream open reading frames. These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation.
Year of Publication2005
JournalNature
Volume438
Issue7071
Pages1105-15
Date Published (YYYY/MM/DD)2005/12/22
ISSN Number0028-0836
DOI10.1038/nature04341
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/16372000?dopt=Abstract