A robust small-molecule microarray platform for screening cell lysates.

Chem Biol
Authors
Keywords
Abstract

Herein we report the expanded functional group compatibility of small-molecule microarrays to include immobilization of primary alcohols, secondary alcohols, phenols, carboxylic acids, hydroxamic acids, thiols, and amines on a single slide surface. Small-molecule "diversity microarrays" containing nearly 10,000 known bioactive small molecules, natural products, and small molecules originating from several diversity-oriented syntheses were produced by using an isocyanate-mediated covalent capture strategy. Selected printed bioactive compounds were detected with antibodies against compounds of interest. The new surface of the diversity microarrays is highly compatible with approaches involving cellular lysates. This feature has enabled a robust, optimized screening methodology using cellular lysates, allowing the detection of specific interactions with a broad range of binding affinity by using epitope-tagged or chimeric fluorescent proteins without prior purification. We believe that this expanded research capability has considerable promise in biology and medicine.

Year of Publication
2006
Journal
Chem Biol
Volume
13
Issue
5
Pages
493-504
Date Published
2006 May
ISSN
1074-5521
URL
DOI
10.1016/j.chembiol.2006.03.004
PubMed ID
16720270
Links
Grant list
GM38627 / GM / NIGMS NIH HHS / United States
R01-AR049832 / AR / NIAMS NIH HHS / United States