Scientific Publications

A method for high-throughput gene expression signature analysis.

Publication TypeJournal Article
AuthorsPeck, D., Crawford ED, Ross KN, Stegmaier K., Golub T. R., and Lamb J.
AbstractGenome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.
Year of Publication2011
JournalGenome biology
Volume7
Issue7
PagesR61
Date Published (YYYY/MM/DD)2011/12/08
ISSN Number1465-6906
DOI10.1186/gb-2006-7-7-r61
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/16859521?dopt=Abstract