A method for high-throughput gene expression signature analysis.

Genome Biol
Authors
Keywords
Abstract

Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.

Year of Publication
2006
Journal
Genome Biol
Volume
7
Issue
7
Pages
R61
Date Published
2006
ISSN
1474-760X
URL
DOI
10.1186/gb-2006-7-7-r61
PubMed ID
16859521
PubMed Central ID
PMC1779561
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