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Genome-scale loss-of-function screening with a lentiviral RNAi library.
| Publication Type | Journal Article |
| Authors | Root, DE, Hacohen N., Hahn WC, Lander E. S., and Sabatini DM |
| Abstract | The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens. |
| Year of Publication | 2006 |
| Journal | Nature methods |
| Volume | 3 |
| Issue | 9 |
| Pages | 715-9 |
| Date Published (YYYY/MM/DD) | 2006/09/01 |
| ISSN Number | 1548-7091 |
| DOI | 10.1038/nmeth924 |
| PubMed | http://www.ncbi.nlm.nih.gov/pubmed/16929317?dopt=Abstract |
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