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Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma.

Publication TypeJournal Article
AuthorsStegmaier, K., Wong JS, Ross KN, Chow KT, Peck D., Wright RD, Lessnick SL, Kung AL, and Golub T. R.
AbstractThe presence of tumor-specific mutations in the cancer genome represents a potential opportunity for pharmacologic intervention to therapeutic benefit. Unfortunately, many classes of oncoproteins (e.g., transcription factors) are not amenable to conventional small-molecule screening. Despite the identification of tumor-specific somatic mutations, most cancer therapy still utilizes nonspecific, cytotoxic drugs. One illustrative example is the treatment of Ewing sarcoma. Although the EWS/FLI oncoprotein, present in the vast majority of Ewing tumors, was characterized over ten years ago, it has never been exploited as a target of therapy. Previously, this target has been intractable to modulation with traditional small-molecule library screening approaches. Here we describe a gene expression-based approach to identify compounds that induce a signature of EWS/FLI attenuation. We hypothesize that screening small-molecule libraries highly enriched for FDA-approved drugs will provide a more rapid path to clinical application.
Year of Publication2007
JournalPLoS medicine
Volume4
Issue4
Pagese122
Date Published (YYYY/MM/DD)2007/04/01
ISSN Number1549-1277
DOI10.1371/journal.pmed.0040122
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/17425403?dopt=Abstract