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Small-molecule reagents for cellular pull-down experiments.
| Publication Type | Journal Article |
| Authors | Wang, X., Imber BS, and Schreiber SL |
| Abstract | Affinity purification of interacting proteins from cellular extracts is a powerful technique for identifying the cellular targets of small molecules. Affinity matrix-based small-molecule reagents are usually prepared by conjugating small molecules of interest to a solid support such as agarose. This protocol describes an efficient and robust method to immobilize small molecules containing a primary alcohol, a common functional group in small molecules, especially in small molecules prepared using diversity-oriented synthesis. This method comprises one element of a systematic approach to the target identification problem in chemical biology. |
| Year of Publication | 2008 |
| Journal | Bioconjugate chemistry |
| Volume | 19 |
| Issue | 3 |
| Pages | 585-7 |
| Date Published (YYYY/MM/DD) | 2008/03/01 |
| ISSN Number | 1043-1802 |
| DOI | 10.1021/bc700297j |
| PubMed | http://www.ncbi.nlm.nih.gov/pubmed/18193824?dopt=Abstract |
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