Scientific Publications

Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.

Publication TypeJournal Article
AuthorsNusbaum, C., Ohsumi TK, Gomez J., Aquadro J., Victor TC, Warren RM, Hung DT, Birren BW, Lander E. S., and Jaffe DB
AbstractOur variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected approximately 98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives.
Year of Publication2009
JournalNature methods
Volume6
Issue1
Pages67-9
Date Published (YYYY/MM/DD)2009/01/01
ISSN Number1548-7091
DOI10.1038/nmeth.1286
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/19079253?dopt=Abstract