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Scientific Publications
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Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.
| Publication Type | Journal Article |
| Authors | Nusbaum, C., Ohsumi TK, Gomez J., Aquadro J., Victor TC, Warren RM, Hung DT, Birren BW, Lander E. S., and Jaffe DB |
| Abstract | Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected approximately 98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives. |
| Year of Publication | 2009 |
| Journal | Nature methods |
| Volume | 6 |
| Issue | 1 |
| Pages | 67-9 |
| Date Published (YYYY/MM/DD) | 2009/01/01 |
| ISSN Number | 1548-7091 |
| DOI | 10.1038/nmeth.1286 |
| PubMed | http://www.ncbi.nlm.nih.gov/pubmed/19079253?dopt=Abstract |
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