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Bead-based profiling of tyrosine kinase phosphorylation identifies SRC as a potential target for glioblastoma therapy.

Publication TypeJournal Article
AuthorsDu, J., Bernasconi P., Clauser KR, Mani D. R., Finn SP, Beroukhim R., Burns M., Julian B., Peng XP, Hieronymus H., Maglathlin RL, Lewis TA, Liau LM, Nghiemphu P., Mellinghoff IK, Louis DN, Loda M., Carr SA, Kung AL, and Golub T. R.
AbstractThe aberrant activation of tyrosine kinases represents an important oncogenic mechanism, and yet the majority of such events remain undiscovered. Here we describe a bead-based method for detecting phosphorylation of both wild-type and mutant tyrosine kinases in a multiplexed, high-throughput and low-cost manner. With the aim of establishing a tyrosine kinase-activation catalog, we used this method to profile 130 human cancer lines. Follow-up experiments on the finding that SRC is frequently phosphorylated in glioblastoma cell lines showed that SRC is also activated in primary glioblastoma patient samples and that the SRC inhibitor dasatinib (Sprycel) inhibits viability and cell migration in vitro and tumor growth in vivo. Testing of dasatinib-resistant tyrosine kinase alleles confirmed that SRC is indeed the relevant target of dasatinib, which inhibits many tyrosine kinases. These studies establish the feasibility of tyrosine kinome-wide phosphorylation profiling and point to SRC as a possible therapeutic target in glioblastoma.
Year of Publication2009
JournalNature biotechnology
Volume27
Issue1
Pages77-83
Date Published (YYYY/MM/DD)2009/01/01
ISSN Number1087-0156
DOI10.1038/nbt.1513
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/19098899?dopt=Abstract