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SNP genotyping using the Sequenom MassARRAY iPLEX platform.
| Publication Type | Journal Article |
| Authors | Gabriel, S., Ziaugra L., and Tabbaa D. |
| Abstract | The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele. |
| Year of Publication | 2009 |
| Journal | Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] |
| Volume | Chapter 2 |
| Pages | Unit 2.12 |
| Date Published (YYYY/MM/DD) | 2009/01/01 |
| ISSN Number | 1934-8266 |
| DOI | 10.1002/0471142905.hg0212s60 |
| PubMed | http://www.ncbi.nlm.nih.gov/pubmed/19170031?dopt=Abstract |
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