Scientific Publications

SNP genotyping using the Sequenom MassARRAY iPLEX platform.

Publication TypeJournal Article
AuthorsGabriel, S., Ziaugra L., and Tabbaa D.
AbstractThe method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.
Year of Publication2009
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeChapter 2
PagesUnit 2.12
Date Published (YYYY/MM/DD)2009/01/01
ISSN Number1934-8266
DOI10.1002/0471142905.hg0212s60
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/19170031?dopt=Abstract