Scientific Publications

Introduction: cell-based assays for high-throughput screening.

Publication TypeJournal Article
AuthorsAn, WF, and Tolliday NJ
AbstractCell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques described in this book.
Year of Publication2012
JournalMethods in molecular biology (Clifton, N.J.)
Volume486
Pages1-12
Date Published (YYYY/MM/DD)2012/01/09
ISSN Number1064-3745
DOI10.1007/978-1-60327-545-3_1
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/19347612?dopt=Abstract