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Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts.

Publication TypeJournal Article
AuthorsLevin, JZ, Berger MF, Adiconis X., Rogov P., Melnikov A., Fennell T., Nusbaum C., Garraway LA, and Gnirke A.
AbstractTargeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.
Year of Publication2012
JournalGenome biology
Volume10
Issue10
PagesR115
Date Published (YYYY/MM/DD)2012/01/09
ISSN Number1465-6906
DOI10.1186/gb-2009-10-10-r115
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/19835606?dopt=Abstract