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Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution.
| Publication Type | Journal Article |
| Authors | Gu, H., Bock C., Mikkelsen TS, Jäger N., Smith ZD, Tomazou E., Gnirke A., Lander E. S., and Meissner A. |
| Abstract | Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples. |
| Year of Publication | 2010 |
| Journal | Nature methods |
| Volume | 7 |
| Issue | 2 |
| Pages | 133-6 |
| Date Published (YYYY/MM/DD) | 2010/02/01 |
| ISSN Number | 1548-7091 |
| DOI | 10.1038/nmeth.1414 |
| PubMed | http://www.ncbi.nlm.nih.gov/pubmed/20062050?dopt=Abstract |
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