Scientific Publications

Cell-based assays for high-throughput screening.

Publication TypeJournal Article
AuthorsAn, WF, and Tolliday N.
AbstractCell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS. Approaches for successful experimental design and execution of cell-based screens are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques used.
Year of Publication2010
JournalMolecular biotechnology
Volume45
Issue2
Pages180-6
Date Published (YYYY/MM/DD)2010/06/01
ISSN Number1073-6085
DOI10.1007/s12033-010-9251-z
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/20151227?dopt=Abstract