Scientific Publications

Densely interconnected transcriptional circuits control cell states in human hematopoiesis.

Publication TypeJournal Article
AuthorsNovershtern, N., Subramanian A., Lawton LN, Mak RH, Haining WN, McConkey ME, Habib N., Yosef N., Chang CY, Shay T., Frampton GM, Drake AC, Leskov I., Nilsson B., Preffer F., Dombkowski D., Evans JW, Liefeld T., Smutko JS, Chen J., Friedman N., Young RA, Golub T. R., Regev A., and Ebert BL
AbstractThough many individual transcription factors are known to regulate hematopoietic differentiation, major aspects of the global architecture of hematopoiesis remain unknown. Here, we profiled gene expression in 38 distinct purified populations of human hematopoietic cells and used probabilistic models of gene expression and analysis of cis-elements in gene promoters to decipher the general organization of their regulatory circuitry. We identified modules of highly coexpressed genes, some of which are restricted to a single lineage but most of which are expressed at variable levels across multiple lineages. We found densely interconnected cis-regulatory circuits and a large number of transcription factors that are differentially expressed across hematopoietic states. These findings suggest a more complex regulatory system for hematopoiesis than previously assumed.
Year of Publication2011
JournalCell
Volume144
Issue2
Pages296-309
Date Published (YYYY/MM/DD)2011/01/21
ISSN Number0092-8674
DOI10.1016/j.cell.2011.01.004
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/21241896?dopt=Abstract