Scientific Publications

Glioblastoma-Derived Epidermal Growth Factor Receptor Carboxyl-Terminal Deletion Mutants Are Transforming and Are Sensitive to EGFR-Directed Therapies.

Publication TypeJournal Article
AuthorsCho, J., Pastorino S., Zeng Q., Xu X., Johnson W., Vandenberg S., Verhaak R., Cherniack AD, Watanabe H., Dutt A., Kwon J., Chao YS, Onofrio RC, Chiang D., Yuza Y., Kesari S., and Meyerson M.
AbstractGenomic alterations of the epidermal growth factor receptor (EGFR) gene play a crucial role in pathogenesis of glioblastoma multiforme (GBM). By systematic analysis of GBM genomic data, we have identified and characterized a novel exon 27 deletion mutation occurring within the EGFR carboxyl-terminus domain (CTD), in addition to identifying additional examples of previously reported deletion mutations in this region. We show that the GBM-derived EGFR CTD deletion mutants are able to induce cellular transformation in vitro and in vivo in the absence of ligand and receptor autophosphorylation. Treatment with the EGFR-targeted monoclonal antibody, cetuximab, or the small molecule EGFR inhibitor, erlotinib, effectively impaired tumorigenicity of oncogenic EGFR CTD deletion mutants. Cetuximab in particular prolonged the survival of intracranially xenografted mice with oncogenic EGFR CTD deletion mutants, compared with untreated control mice. Therefore, we propose that erlotinib and, especially, cetuximab treatment may be a promising therapeutic strategy in GBM patients harboring EGFR CTD deletion mutants. Cancer Res; 71(24); 7587-96. ©2011 AACR.
Year of Publication2011
JournalCancer research
Volume71
Issue24
Pages7587-96
Date Published (YYYY/MM/DD)2011/12/15
ISSN Number0008-5472
DOI10.1158/0008-5472.CAN-11-0821
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/22001862?dopt=Abstract