Broad Workshops

BroadE workshops bring researchers in the extended Broad community together so they can learn from one another. BroadE workshops (the 'E' stands for education) offer insights and share hands-on training in breakthrough technologies, high-throughput methods, and computational tools not typically found in conventional research labs. Through this ongoing series, which is open to Broad staff and to researchers at MIT, Harvard and Harvard-affiliated hospitals, the Broad community hopes to extend the impact of its science and openly share new methods.

 

New Registration System

It’s official — BroadE is a rousing success! In fact, demand is so high that our workshops quickly reach capacity, sometimes only minutes after being posted.

To meet this overwhelming demand and promote access to all in our community, we will repeat our most popular workshops during the coming year.

In addition, we will implement a new registration system for BroadE workshops occurring in June 2013 and later. Going forward, when a BroadE workshop is announced, we will also open a one-week application period. Participants will be selected from the pool of applicants who register during this period. In selecting participants from this pool, we will consider:

  • the applicant’s home institution
  • the applicant’s lab
  • whether the applicant has participated in previous workshops

This new registration practice will help us to ensure that BroadE workshops reach a diverse, balanced group of Broadies.

 

Upcoming Workshops

2014-2015        
Charting the Epigenome with ChIP - Basic Analyses of ChIP-Seq Data
ChIP (chromatin immunoprecipitation) is a very powerful technique that enables the localization of proteins on DNA throughout the genome. The technique relies on the selective enrichment of a chromatin fraction containing a specific antigen, by immunoprecipitation. Antibodies that recognize a protein or protein modification are used to capture the chromatin (protein - DNA complex), and in the contemporary method of ChIP-Seq, Nextgen sequencing libraries are derived from the recovered DNA. The libraries are sequenced and the recovered sequences are aligned to a genomic scaffold to map the locations of the antigen recognized by the antibody. The ChIP technique can be used in any area of research to further elucidate gene function and regulation in their native state.

Application of ChIP to the genome wide localization of DNA binding proteins, transcription factors, chromatin modifying enzymes, and histone modifications has helped develop an understanding of the mechanisms for the regulation of chromatin organization. These methods have contributed to our understanding of embryogenesis and tissue specific cellular differentiation, while aberrant chromatin structure is associated with development disorders and other diseases, such as cancer.

The workshop will cover the following topics:
  1. Introduction to chromatin, ChIP and usages of the method.
  2. Overview of genomic approaches to map in-vivo chromatin structure (ChIP-Seq).
  3. Detailed description of genome-wide mapping of chromatin by ChIP-Seq - e.g., analysis methods, validations, sample requirements, reproductibility, quality assessment and materials validation.
  4. Use of visualization tools such as IGV and UCSC Genome Browser.
  5. Major scientific discoveries stemming from charting of in-vivo chromatin maps - chromatin states, enhancer mapping, organization of the chromatin regulators, dynamics of transcription factors binding.
  May 9   Registration has closed
   
CRISPR
 
  May 13   Description and registration coming soon!
   
LINCS/CMAP
 
  May 30   Description and registration coming soon!
   
Single-Cell Genomics
 
  June 2   Description and registration coming soon!
   
CellProfiler
This workshop will instruct participants in the use of CellProfiler, an open-source, freely-downloadable software package designed for large-scale, automated phenotypic image analysis. Attendees are encouraged to contribute sample images from their assays as part of the demonstration. We will also briefly discuss the basic principles of supervised machine learning in order to score phenotypes where phenotypic differences between samples are not visible by eye.
  June 6   Registration coming soon!
   
Visual Representation for Exploring and Explaining Scientific Data
 
  June 11   Description and registration coming soon!
   
IGV
 
  June 27   Description and registration coming soon!
   
Primer on Medical and Population Genetics
The Primer on Medical and Population Genetics series includes informal discussions of basic topics relevant to medical and population genetics by speakers drawn from across the Broad community. The target audience includes technicians, grad students, postdocs and established investigators just entering the field of complex trait genetics with the goal of bringing all up to speed on basic principles of human genetic variation, genotyping and sequencing methods, statistics, and analysis.

Please note: When the weekly MPG meeting is cancelled (due to weather, conflicting events, etc.) Primers are also cancelled.
  Thursdays
September - January

 
  No Registration Necessary