Sorry to post such a simple question but I seem to be at my wits end. Base Recalibrator keeps giving me this error:
Why isn't HiSeq2000 recognized as Illumina?
I'm trying to run CountCovariates and getting the error as :
" java.lang.IllegalStateException: This method hasn't been implemented yet for GAIIx "
I have used following program arguments as given below :
Program Args: -T CountCovariates -R /home/adlab/ngs_data/databases/human_g1k_v37.fasta -I D5_b37_aligned_sorted_realn_DupRm.bam -knownSites:dbsnp,VCF /home/adlab/ngs_data/databases/dbsnp_132.b37.vcf -recalFile D5_b37_aligned_sorted_realn_DupRm_countcovariates.csv -cov ReadGroupCovariate -cov QualityScoreCovariate -cov CycleCovariate -cov DinucCovariate -standard
To trouble shoot it I found that I have added the read groups in bwa sampe by setting platform info as "GAIIx" as given below and it's root for the error.
"@RG\tID:Clone-D5\tPL:GAIIx\tLB:LIB-RDT\tSM:UNKNOWN\I:200"
What's the exact error in my argument list / read group syntax and What could be the cause of the error ? kindly help.