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Hi, I use GATK for Variant Call in an Investigation Unit. When I use RealignerTargetCreator, I get an error: I'm using a wrong encoding for quiality scores. Which encoding (sanger, illumina, solexa) is ok to use with GATK? Do you have any tool to convert to that encoding? Thank you

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Hi all, I am working with GATK on illumina data that was created from yeasts (SK1 strain). Since it is sequencing of a colony and not the exact same organism, I am using a filter that I wrote on the ratio of the reads that support the alternative allele. Is anyone else using something similar? Is there a build-in filter like that?