I have seen the definition of strand bias on this site (below) but I need a little clarification. Does the FS filter (a) highlight instances where reads are only present on a single strand and contain a variant (as may occur toward the end of exome capture regions) or does it (b) specifically look for instances where there are reads on both strands but the variant allele is disproportionately represented on one strand (as might be indicative of a false positive), or does it (c) do both?
I had thought it did (b) but have encountered some disagreement.
** How much evidence is there for Strand Bias (the variation being seen on only the forward or only the reverse strand) in the reads? Higher SB values denote more bias (and therefore are more likely to indicate false positive calls.
I am filtering looking for rare variants and found some frameshift variants in an interesting gene. Some of them are noted as PASS in the QC column of the VCF and some are noted as Indel_FS . What exactly does that second notation mean? I am almost positive that these will validate given how they segregate in my subjects.